《分子生物学实验指导（英文版）》以科研思路的培养为基本出发点，以真核生物基因为实验材料，涵盖DNA提取、分子克隆、蛋白质表达纯化、RNA提取、表达谱分析、转录水平调控分析方法等多个基础实验。各实验既可以单独开设，也可以将前后联系的独立实验整合为综合实验。每个实验分为背景知识与概念、操作步骤、注意事项以及思考题等内容，并在书后附仪器的使用说明、基本操作的介绍，网络资源使用简介等补充知识。全书双色印刷。
- 前言
- Experiment 1 Preparation of Plasmid DNA
- Experiment 2 Restriction Enzyme Digestion and Agarose Gel Electrophoresis
- Experiment 3 Polymerase Chain Reaction (PCR)
- Experiment 4 DNA Purification and Construction of Recombinant DNA
- Experiment 5 Preparation of Competent E. coli Cells
- Experiment 6 Transformation of Recombinant DNA
- Experiment 7 Identification and Characterization of Positive Clones
- Experiment 8 Isolation of Total RNA from Animal Tissues
- Experiment 9 Molecular Hybridization
- Experiment 10 Reverse Transcription – Polymerase Chain Reaction (RT-PCR) and TA-cloning
- Experiment 11 Expression and Purification of a Fusion Protein from E. coli
- Experiment 12 Electrophoretic Mobility Gel Shift Assay (EMSA) to Detect DNA-Protein Interactions
- Appendix I Brief Introduction to the NCBI Database
- Appendix II Vector Information
- Appendix III Instructions on How to Use the Equipment
- References
- 版权
