本书根据中国药科大学高质量发展、“双一流”建设和化学基础课程教学改革的需要编写而成，侧重药学研究领域仪器分析实验技能和科研思维的培养，同时关注了仪器分析实验新技术，力求培养学生的实验动手能力和分析问题、解决问题的能力。
本书分为6章，包括仪器分析实验基础知识、电化学分析、光谱分析、色谱分析、色谱-质谱联用法及设计类实验，共45个实验，以及6个设计实验选题。内容采用中英文对照，便于双语教学。
本书可作为高等学校药学类专业本科生仪器分析实验课程教材，也可供医学、食品及化学化工等相关学科实验教学和分析工作者的参考用书。
- 目录
- 前辅文
- Chapter 1 Basic Knowledge of Instrumental Analysis Experiment
- 1.1 General Knowledge of Laboratory Safety
- 1.2 Experimental Report
- 1.3 Disposal of Chemical Laboratory Waste
- 1.4 Colored Cylinder Mark for Gases and the Precautions of Gas Cylinders for Use in Laboratory
- 1.5 Data Processing Software Commonly Used in Instrumental Analysis Experiments: Introduction to Origin 2018 Software
- Chapter 2 Electrochemical Analysis
- Experiment 2.1 Determination of pH Values of Liquid Pharmaceutical Preparations
- Experiment 2.2 Potentiometric Titration Analysis of Phosphoric Acid and Determination of Dissociation Constant
- Experiment 2.3 Determination of Procaine Hydrochlorid by Dead-Stop Titration
- Experiment 2.4 Determination of Caffeine by Potentiometric Titration
- Experiment 2.5 Determination of Fluoride Ion in Drinking Water by Fluoride Ion Selective Electrode
- Experiment 2.6 Determination of Water Content in Antibiotic Drugs
- Chapter 3 Spectral Analysis
- Experiment 3.1 Solvent Effect of Ultraviolet Absorption Spectra of Organic Compounds
- Experiment 3.2 Determination of the Absorption Coefficient of API
- Experiment 3.3 Identification and Determination of Vitamin B, Injection by Ultraviolet-visible Spectrophotometry
- Experiment 3.4 Condition Optimization for Colorimetric Determination of Iron with 1, 10-Phenanthroline
- Experiment 3.5 Colorimetric Determination of Trace Iron in Water with 1, 10-Phenanthroline
- Experiment 3.6 Determination of Sulfamethoxazol in the Compound Sulfamethoxazole Tablets by the Dual-Wavelength Method
- Experiment 3.7 Determination of Adrenalone in Adrenaline by Ultraviolet-visible Spectrophotometry
- Experiment 3.8 Preparation and Spectral Determination of Samples with Different States in Infrared Spectrophotometry
- Experiment 3.9 Determination of the Drug Chemical Structure by Infrared Spectrophotometry
- Experiment 3.10 Determination of Magnesium in Tap Water by Atomic Absorption Spectrophotometry
- Experiment 3.11 Determination of Calcium in Calcium Gluconate Oral Solution by Atomic Absorption Spectrometry
- Experiment 3.12 Determination of Cadmium and Lead in Salvia miltiorrhiza by Inductively Coupled Plasma-Atomic Emission Spectrometry
- Experiment 3.13 Determination of Heavy Metals in Soil by Inductively Coupled Plasma-Atomic Emission Spectrometry
- Experiment 3.14 Excitation and Emission Spectra of Anthracene by Fluorescence Spectroscopy
- Experiment 3.15 Determination of Acetylsalicylic Acid and Salicylic Acid in Aspirin Tablets by Fluorescence Analysis
- Experiment 3.16 Determination of Aluminum Ion in Water by Fluorescence Analysis
- Experiment 3.17 Assay of Reserpine Tablets by Fluorescence Analysis
- Experiment 3.18 Determination of Quinine in Tonic Water by Standard Addition Method
- Experiment 3.19 Structure Elucidation of Aspirin by 'H Nuclear Magnetic Resonance Spectroscopy
- Chapter 4 Chromatographic Analysis
- Experiment 4.1 Activity Determination of Silica Gel Adhesive Thin Layer
- Experiment 4.2 Identification of Buguzhi (Fructus Psoraleae) by Thin Layer Chromatography
- Experiment 4.3 Separation and Identification of Methionine and Glycine by Paper Chromatography
- Experiment 4.4 Determination of Dead Time and Content of Alkane Mixture with Normalization Method by Gas Chromatography
- Experiment 4.5 Effect of Mobile Phase Velocity on Column Efficiency in Gas Chromatography
- Experiment 4.6 Determination of Alcohol in Tincture by Gas Chromatography
- Experiment 4.7 Determination of Residual Solvent by Temperature Programmed Capillary Gas Chromatography
- Experiment 4.8 Evaluation of the High Performance Liquid Chromatography Performance and Calculation of the Chromatographic Parameters
- Experiment 4.9 Determination of Salicylic Acid Impurity in Aspirin by High Performance Liquid Chromatography
- Experiment 4.10 Determination of Caffeine in Food by High Performance Liquid Chromatography
- Experiment 4.11 Determination of Nandrolone Phenylpropionate by High Performance Liquid Chromatography
- Experiment 4.12 Determination of Sulfamethoxazole and Trimethoprim in Compound Sulfamethoxazole Tablet by Capillary Electrophoresis
- Experiment 4.13 Separation of Chiral Drug Enantiomer by Capillary Zonc Electrophoresis
- Chapter 5 Chromatography-Mass Spectrometry
- Experiment 5.1 Performance Verification of Gas Chromatography-Mass Spectrometer
- Experiment 5.2 Performance Verification of High Performance Liquid Chromatography-Mass Spectrometer
- Experiment 5.3 Determination of Eugenol in Caryophylli Flos by Gas Chromatography-Mass Spectrometr
- Experiment 5.4 Determination of p-Phenylenediamine in Hair Dyes by Gas Chromatography-Mass Spectrometry
- Experiment 5.5 Determination of Paracetamol by Liquid Chromatography-Mass Spectrometry
- Experiment 5.6 Determination of Sulfonamides by Liquid Chromatography-Mass Spectrometry
- Experiment 5.7 Determination of Isosorbide-5-Mononitrate in Human Plasma by Ultra-high Performance Liquid Chromatography-Mass Spectrometry
- Chapter 6 Designing Experiment
- 6.1 Overview
- 6.2 General Steps of Designing for the Analysis Scheme
- 6.3 Analysis of Scheme Design Cases
- 6.4 Typical Example of Designing Experiment
- 6.5 Reference Topics of Designing Experiment
- Reference
- Appendix
