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EXPERIMENT AND GUIDE FOR MEDICAL BIOLOGY
《医学生物学实验（英文版）》由2部分组成。第一部分概括地介绍医学生物学实验的目的、实验室规范、实验安全、常用实验室仪器、实验方法以及实验数据的评价。第二部分包括25个实验，包括生物分子的分析、细胞和超微结构的分析、分子生物学实验、医学遗传学实验、实验技术在医学研究中的应用等。书中所介绍的所有实验都是经过验证的，而且学生在正常实验课期间均可完成。
作者：
侯燕芝
定价：
20.00元
出版时间：
2012-02-16
ISBN：
978-7-04-033433-3
物料号：
33433-00
读者对象：
高等教育
一级分类：
医药
二级分类：
基础医学
重点项目：
暂无
版面字数：
150千字
开本：
16开
全书页数：
108页
装帧形式：
平装
- Chapter 1 Introduction to theM edicalBiology Laboratory
- 1.1 Objectives of a Teaching Medical Biology Laboratory
- 1.2 Laboratory Rules
- 1.3 Laboratory Safety
- 1.4 Common Laboratory Equipments
- 1.5 Experimental Methods in Medical Biology
- 1.6 Evaluation of Experimental Data and Laboratory Reports
- Chapter 2 Experiments in M edicalBiology
- Experiment 1 The Use of Glass Pipets and Pipettors
- Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins
- Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie Brilliant Blue Assay
- Experiment 4 Quantitative Determination of Proteins by Folin‐phenol Assay
- Experiment 5 Determination of Proteins by UV Absorption
- Experiment 6 Separation of Amino Acid Mixtures by Ion‐exchange Chromatography
- Experiment 7 Separation of Serum γ‐globulins by Ion‐exchange Chromatography
- Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis
- Experiment 9 Separation and Purity Determination of Serum γ‐globulins by SDS‐polyacrylamide Gel Electrophoresis
- Experiment 10 Determination of Serum Glutamic‐pyruvic Transaminase Activity
- Experiment 11 Determination of Serum Urea Nitrogen Content
- Experiment 12 Determination of Blood Glucose Concentration
- Experiment 13 Subculture of Adhesion Cells
- Experiment 14 Counting and Determination of Live／Dead Cells
- Experiment 15 Preparation of Cell Samples for Observation of Cell Morphologies
- Experiment 16 Mitosis and Meiosis of Cells
- Experiment 17 Intracellular Distribution of Biomacromolecules
- Experiment 18 Isolation of Subcellular Components
- Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells
- Experiment 20 Banding Pattern of Human Chromosomes
- Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells
- Experiment 22 Isolation ,Amplification and Identification of the β‐actin Gene Fragment from Mouse Liver Cells
- Experiment 23 Purification of Target DNA and Generation of Recombinant DNA
- Experiment 24 Transformation and Screening of Recombinant DNA
- Experiment 25 Determination of Serum Proteins Using Western Blotting
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