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EXPERIMENT AND GUIDE FOR MEDICAL BIOLOGY


作者:
侯燕芝
定价:
20.00元
ISBN:
978-7-04-033433-3
版面字数:
150千字
开本:
16开
全书页数:
108页
装帧形式:
平装
重点项目:
暂无
出版时间:
2012-02-16
读者对象:
高等教育
一级分类:
医药
二级分类:
基础医学

《医学生物学实验(英文版)》由2部分组成。第一部分概括地介绍医学生物学实验的目的、实验室规范、实验安全、常用实验室仪器、实验方法以及实验数据的评价。第二部分包括25个实验,包括生物分子的分析、细胞和超微结构的分析、分子生物学实验、医学遗传学实验、实验技术在医学研究中的应用等。书中所介绍的所有实验都是经过验证的,而且学生在正常实验课期间均可完成。

  • Chapter 1 Introduction to theM edicalBiology Laboratory
    • 1.1 Objectives of a Teaching Medical Biology Laboratory
    • 1.2 Laboratory Rules
    • 1.3 Laboratory Safety
    • 1.4 Common Laboratory Equipments
    • 1.5 Experimental Methods in Medical Biology
    • 1.6 Evaluation of Experimental Data and Laboratory Reports
  • Chapter 2 Experiments in M edicalBiology
    • Experiment 1 The Use of Glass Pipets and Pipettors
    • Experiment 2 Amphoteric Ionization and Isoelectric Point Determination of Proteins
    • Experiment 3 Quantitative Determination of Protein Concentrations by Coomassie Brilliant Blue Assay
    • Experiment 4 Quantitative Determination of Proteins by Folin‐phenol Assay
    • Experiment 5 Determination of Proteins by UV Absorption
    • Experiment 6 Separation of Amino Acid Mixtures by Ion‐exchange Chromatography
    • Experiment 7 Separation of Serum γ‐globulins by Ion‐exchange Chromatography
    • Experiment 8 Separation of Serum Proteins by Cellulose Acetate Membrane Electrophoresis
    • Experiment 9 Separation and Purity Determination of Serum γ‐globulins by SDS‐polyacrylamide Gel Electrophoresis
    • Experiment 10 Determination of Serum Glutamic‐pyruvic Transaminase Activity
    • Experiment 11 Determination of Serum Urea Nitrogen Content
    • Experiment 12 Determination of Blood Glucose Concentration
    • Experiment 13 Subculture of Adhesion Cells
    • Experiment 14 Counting and Determination of Live/Dead Cells
    • Experiment 15 Preparation of Cell Samples for Observation of Cell Morphologies
    • Experiment 16 Mitosis and Meiosis of Cells
    • Experiment 17 Intracellular Distribution of Biomacromolecules
    • Experiment 18 Isolation of Subcellular Components
    • Experiment 19 Preparation of Chromosome Samples from Mouse Bone Marrow Cells
    • Experiment 20 Banding Pattern of Human Chromosomes
    • Experiment 21 Colony Forming Efficiency and Total Protein Analysis of Tumor Cells
    • Experiment 22 Isolation ,Amplification and Identification of the β‐actin Gene Fragment from Mouse Liver Cells
    • Experiment 23 Purification of Target DNA and Generation of Recombinant DNA
    • Experiment 24 Transformation and Screening of Recombinant DNA
    • Experiment 25 Determination of Serum Proteins Using Western Blotting

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